《植物生理学报》 2013, 49(9): 968-972

无籽罗汉果的组织培养和快速繁殖

陈继富*

湘西民族职业技术学院生物工程系, 湖南吉首416000

通信作者：陈继富；E-mail: chenjifu1965@163.com；Tel: 0743-8535355

摘　要：

以无籽罗汉果优良株系的幼嫩茎段为试验材料, 经消毒处理后, 剪成带一个腋芽的茎段, 在MS+6-BA 0.5 mg·L^-1+NAA 0.05 mg·L^-1培养基上进行培养, 获得无菌芽苗, 再以无菌芽苗的单芽茎段为外植体, 建立无籽罗汉果的组培快繁体系。结果表明, 最佳继代增殖培养基为MS+6-BA 0.5 mg·L^-1+IBA 0.2 mg·L^-1+GA3 0.03 mg·L^-1, 30 d的增殖系数为16.4; 芽苗伸长的最适培养基为MS+6-BA 0.05 mg·L^-1+IBA 0.1 mg·L^-1+GA3 0.1 mg·L^-1; 芽苗生根的最适培养基为1/2MS+IBA 0.5 mg·L^-1; 炼苗后, 移入蛭石:珍珠岩:熟土=1:1:2 (V/V/V)的基质中, 成活率达98.1%。该体系的建立为无籽罗汉果规模化生产提供了技术平台。

关键词：罗汉果; 组织培养; 快速繁殖

收稿：2013-07-11   修定：2013-08-02

资助：湖南省科技厅湘西专项(2012FJ4440)和湘西土家族苗族自治州科技计划项目(11XI012)。

Tissue Culture and Rapid Propagation of Seedless Siraitia grosvenorii

CHEN Ji-Fu*

Bioengineering Department, Xiangxi National Vocation Technical College, Jishou, Hunan 416000, China

Corresponding author: CHEN Ji-Fu; E-mail: chenjifu1965@163.com; Tel: 0743-8535355

Abstract:

In order to study the tissue culture and rapid propagation technique of triploid seedless system of Siraitia grosvenorii, young stem was used as test materials. After disinfection, the stem segments with one bud were cultured in MS+6-BA 0.5 mg·L^-1+NAA 0.05 mg·L^-1 medium, to obtain the aseptic seedling. Then stems with axillary buds were taken as explants to established the in vitro propagation system of seedless S. grosvenorii. The results show that, the best medium for multiplication was MS+6-BA 0.5 mg·L^-1 +IBA 0.2 mg·L^-1 + GA₃ 0.03 mg·L^-1, the multiplication coefficient was 16.4 after 30 d; the optimum medium for shoot elongation was MS+6-BA 0.05 mg·L^-1+IBA 0.1 mg·L^-1+GA₃ 0.1 mg·L^-1; the optimal medium for rooting was 1/2MS+IBA 0.5 mg·L^-1. After hardening, it was moved into the matrix which volume ratio as vermiculite: perlite:mellow soil=1:1:2, the survival rate was 98.1% after 30 d. These results could provide the technical basis for scale production of seedless plantlets of S. grosvenorii.

Key words: S. grosvenorii; tissue culture; rapid propagation